Camselect NGS for Size selection
Cambrians Bioworks launches Camselect NGS for size specific selection of DNA fragments as applicable in the short read sequencing workflows.
Use Camselect for
– Illumina library preparation
– Oxford nanopore library preparation
– PCR clean up
– Size selection of amplicons above 100bp in size
This is a general overview of the library prep workflows typically implemented using the reagents required for end repair, dA-tailing, adapter ligation and library amplification.
Size selection and Pcr clean are one of the most universally implemented workflow steps that are common across various applications of sequencing.
The chart to the right shows how using Camselect for size selection and PCR clean up can reduce costs of the library prep further down.
A robust quality system ensures uniform size and uniform carboxyl content on the bead with every batch manufactured.
Quality aspects are measured using DLS and binding capacity is validated using in house spectrophotometers.
A 100 bp DNA ladder was used to demonstrate the left side selection performance of Camselect NGS
The results were analysed on an Agilent Tapestation and sample QC was performed on a Qubit system.
A 100bp DNA ladder was used to demonstrate the right side selection performance of Camselect NGS
The results were analysed on an Agilent Tapestation and sample QC was performed on a Qubit system.
