CamSelect NGS
Camselect® NGS beads are capable of selectively binding fragments depending on the reagent-to-sample ratio used, giving the user flexibility to perform left, right or double-sided size selection.
This product is designed for both manual and fully automated purification of DNA and RNA samples,as well as for the purification of PCR products.
Sharp cut-off for size selection
Genomic DNA was extracted from a whole blood sample, using a Qiagen DNeasy Blood and tissue kit with RNAse treatment. The extracted DNA was sheared using Covaris-mediated hydrodynamic shearing. The concentration of the sheared DNA was estimated using Qubit and was found to be 4 ng/μl. In this study, a 50 μl sheared DNA sample was taken for performing the size selection experiments. The results were analysed on an Agilent Tapestation and sample QC was performed on a Qubit system.
Protocol that you already know
Camselect is also applicable for clean up of the DNA and RNA fragments post PCR amplification. This enables removal of all excessive dntps and other elements of reaction mix. With high recovery rates of up to 90%, Camselect is the go to choice for quick column free magnetic stand based recovery. Below is a figure summarising normalised recovery observed for three different PCR fragments.
PCR Clean up
Camselect is also applicable for clean up of the DNA and RNA fragments post PCR amplification. This enables removal of all excessive dntps and other elements of reaction mix. With high recovery rates of up to 90%, Camselect is the go to choice for quick column free magnetic stand based recovery. Below is a figure summarising normalised recovery observed for three different PCR fragments.
Repeatable Performance
Most importantly Camselect also enables automation using any liquid handler platform. Besides this, Camselect also enables users to achieve high recovery consistently, as seen from the repeatability data for two different
PCR products shown below, involving six repeats each.
PCR products shown below, involving six repeats each.
Get free samples
At Cambrian we are building solutions for the next generation diagnostics workflows. Our products enable sample collection and long term storage, NA isolation from difficult samples and library preparation workflows for genomics research.
